Isolation and in vitro Screening of Plant Growth Promoting Rhizospheric Bacteria from Corn (Zea mays var. indentata)

Many farmers use organic fertilizers to preserve the environment, but unfortunately, it’s not as effective chemical fertilizers. In recent years, discovery of plant growth-promoting rhizobacteria (PGPR) has gained some attraction. PGPR have abilities promote growth efficiently, including corn plants. Corn (Zea mays L.) is a staple food needed in large quantities. Our results show that bacteria may potential become for corn. samples were obtained from maize plantations Bunulrejo district, Blimbing, Malang (Republic Indonesia). The bacterial isolates rhizosphere and endophyte area was determined based on several vitro screening results, siderophore production test, indole-3-acetic acid (IAA) phytohormone 1-aminocyclopropane-1-carboxylic (ACC) deaminase phosphate dissolution test. Five contained best PGPR, namely E6.2, E3.1, E4.2, R4.2, R2.1. Later, E6.2 E3.1 known be able fix nitrogen produce siderophores, IAA ACC deaminase. Based sequencing R2.1 isolate considered Bordetella muralis, identified Cellulosimicrobium cellulans, while belonged Serratia nematodiphila. These could used inoculants biofertilizers better agricultural practice.